Journal: Journal of Ovarian Research

Article Title: IL-24 amplifies baicalein-induced immunogenic cell death in ovarian cancer by boosting endoplasmic reticulum stress

doi: 10.1186/s13048-025-01953-3

Figure Lengend Snippet: Baicalein induces IL-24 expression via ER Stress in OC cells. A KEGG pathway enrichment analysis of RNA-Seq data comparing BAI-treated and untreated ID8 cells, highlighting significantly upregulated pathways. B Heatmap displaying differential gene expression within the cytokine–cytokine receptor interaction pathway between BAI and untreated control. C qRT-PCR analysis of Il24 mRNA expression in ID8 cells treated with BAI (100 or 200 μM) for 24 and 48 h. Data are shown as fold change relative to control. D Quantification of Il24 mRNA levels in ID8 cells following treatment with combination of BAI (100 μM) and ER stress pathway inhibitor GSK2656157 (GSK157, 0.5 and 2 μM) for 24 h. Data are representative of two independent experiments with three replicates (C-D). All data are presented as mean ± SEM. * P < 0.05 was considered statistically significant. ** P < 0.01. *** P < 0.001. **** P < 0.0001

Article Snippet: ER stress inhibitor GSK2656157 (GSK157, HY-13820) and antioxidant N-acetylcysteine (NACH, Y-B0215) were obtained from MedChemExpress.

Techniques: Expressing, RNA Sequencing, Gene Expression, Control, Quantitative RT-PCR